TATA Box Binding Protein抗体;TATA Box Binding Protein Antibody—艾普蒂 新品

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     Immunohistochemistry analysis of paraffin-embedded Human Breast Carcinoma using TATA Box Binding Protein antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.    

  
  

The TATA box-binding protein (TBP) is a critical component of the transcription factor TFIID, essential for initiating RNA polymerase II-mediated transcription. It binds specifically to the TATA box, a conserved DNA sequence in gene promoters, facilitating the assembly of the pre-initiation complex. TBP also plays roles in transcription by RNA polymerases I and III, highlighting its broad regulatory importance. Structurally, TBP contains a highly conserved C-terminal domain responsible for DNA binding and protein interactions, while the N-terminal region varies across species and may modulate transcriptional activity.

Antibodies targeting TBP are widely used in molecular biology to study transcriptional mechanisms, protein-DNA interactions, and gene regulation. These antibodies enable detection and quantification of TBP in techniques like Western blotting, immunoprecipitation (ChIP), and immunofluorescence. They are crucial for investigating diseases linked to transcriptional dysregulation, including cancers and neurodegenerative disorders. Researchers often validate TBP antibodies using TBP-deficient cell lines or recombinant protein controls to ensure specificity. Cross-reactivity with TBP orthologs (e.g., human vs. mouse) should be confirmed for experimental models. Some antibodies distinguish post-translational modifications or specific isoforms, aiding functional studies. Commercial TBP antibodies are typically raised in rabbits or mice, with clones like 1TBP18 (mouse monoclonal) being frequently cited. Proper controls, such as siRNA-mediated knockdown, are recommended to confirm signal specificity, as non-specific bands may appear due to TBP's low molecular weight (~38 kDa) and abundant binding partners.