p38抗体;p38 Antibody—艾普蒂

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     Immunohistochemistry analysis of paraffin-embedded Human Lung Carcinoma Tissue using p38 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.    

  
  

The p38 antibody is a crucial tool in molecular biology and biomedical research, targeting members of the p38 mitogen-activated protein kinase (MAPK) family. This family, including isoforms p38α (MAPK14), p38β (MAPK11), p38γ (MAPK12), and p38δ (MAPK13), regulates cellular responses to stress, inflammation, and apoptosis. Discovered in the 1990s, p38 kinases are activated by dual phosphorylation on conserved threonine and tyrosine residues in response to cytokines, environmental stressors, or pathogenic stimuli.

Researchers use p38 antibodies to detect protein expression, phosphorylation status, and localization in various experimental models. These antibodies are essential for studying signaling pathways in diseases like cancer, autoimmune disorders, and neurodegenerative conditions. Specificity varies among commercial antibodies, with some recognizing total p38 (regardless of activation state) and others targeting phosphorylated forms (e.g., p-p38 at Thr180/Tyr182). Validation methods include knockout controls and stimulation-inhibition experiments using known p38 activators (e.g., anisomycin) or inhibitors (e.g., SB203580).

Applications span Western blotting, immunohistochemistry, flow cytometry, and immunofluorescence. Proper antibody selection depends on experimental goals, sample type, and required specificity. Recent advances focus on isoform-specific antibodies to dissect unique biological roles of individual p38 family members. However, cross-reactivity remains a validation challenge, particularly in distinguishing closely related isoforms or phosphorylation states.