alpha Actinin抗体;alpha Actinin Antibody—艾普蒂

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     Immunohistochemistry analysis of paraffin-embedded Human Breast Carrcinoma Tissue using alpha Actinin antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.    

  
  

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     Immunohistochemical analysis of paraffin-embedded Human tonsils using alpha Actinin antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.    

  
  

Alpha-actinin antibodies are essential tools in biomedical research for studying the expression, localization, and function of alpha-actinin proteins. Alpha-actinins are a family of actin-binding proteins belonging to the spectrin superfamily, playing critical roles in cytoskeletal organization, cell adhesion, and mechanotransduction. They exist as antiparallel dimers with an N-terminal actin-binding domain, central spectrin-like repeats, and a C-terminal calmodulin-like domain. Four isoforms (ACTN1-4) are encoded by distinct genes in humans. ACTN1 and ACTN4 are ubiquitously expressed in non-muscle cells, regulating focal adhesions and stress fibers, while ACTN2 and ACTN3 are predominantly found in striated muscle, stabilizing sarcomeric Z-discs.

Antibodies targeting alpha-actinin are widely used to investigate cellular dynamics, particularly in muscle physiology, cancer metastasis, and cardiovascular diseases. In research, they enable techniques like immunofluorescence, Western blotting, and immunohistochemistry to visualize subcellular distribution or quantify protein levels. Muscle-specific alpha-actinin antibodies (e.g., anti-ACTN2) are crucial for diagnosing muscular dystrophies or studying cardiomyopathies. Meanwhile, anti-ACTN4 antibodies are employed in cancer studies due to this isoform’s association with tumor invasion and metastasis.

Commercial alpha-actinin antibodies are typically raised in rabbits, mice, or goats, with monoclonal and polyclonal variants available. Validation often includes knockout cell lines or tissue-specific expression patterns to ensure specificity. These antibodies have become indispensable for unraveling alpha-actinin’s dual roles as a structural scaffold and signaling hub in health and disease.