Thioredoxin抗体;Thioredoxin Antibody—艾普蒂

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     Western blot analysis of Thioredoxin in Jurkat, Hela lysates using Thioredoxin antibody.    

  
  

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     Immunocytochemistry analysis of Thioredoxin (green) in Hela using Thioredoxin antibody,and DAPI(blue).    

  
  

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     Immunohistochemistry analysis of paraffin-embedded Human tonsil using Thioredoxin/TRX antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.    

  
  

Thioredoxin (Trx) antibodies are essential tools in studying the redox-regulating protein thioredoxin, which plays a pivotal role in maintaining cellular redox homeostasis. Thioredoxin, a small (~12 kDa) ubiquitous protein, functions as a disulfide reductase by utilizing its conserved CXXC active site to reduce target proteins. It participates in diverse cellular processes, including antioxidant defense, DNA synthesis, apoptosis regulation, and modulation of transcription factors like NF-κB and AP-1. Dysregulation of Trx expression or activity is linked to cancer, neurodegenerative diseases, and inflammatory conditions, making it a key biomarker and therapeutic target.

Anti-thioredoxin antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect Trx expression and localization in tissues or cell lines. They help researchers investigate Trx's interaction with binding partners like thioredoxin-interacting protein (TXNIP) and its role in oxidative stress responses. Polyclonal and monoclonal variants are available, typically raised in rabbits or mice against full-length Trx or specific epitopes. Some antibodies distinguish between reduced and oxidized Trx forms, aiding in redox state analysis. Validated applications and species reactivity (human, mouse, rat) are critical considerations for experimental design. These antibodies have advanced studies in cancer (e.g., Trx overexpression in tumors) and neurodegeneration (e.g., Alzheimer's disease models), providing insights into disease mechanisms and potential treatments.