Phospho-PERK (Thr982)抗体;Phospho-PERK (Thr982) Antibody—艾普蒂

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     Western blot analysis of Phospho-PERK (Thr982) in A453, AD293, Hela lysates using Phospho-PERK (Thr982) antibody.    

  
  

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     Immunohistochemistry analysis of paraffin-embedded mouse kidney tissue using Phospho-PERK (Thr982) antibody High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.    

  
  

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     Immunohistochemistry analysis of paraffin-embedded rat lung tissue using Phospho-PERK (Thr982) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.    

  
  

The Phospho-PERK (Thr982) antibody is a crucial tool for studying the activation of Protein Kinase R-like Endoplasmic Reticulum Kinase (PERK), a key sensor in the unfolded protein response (UPR) pathway. PERK, encoded by the *EIF2AK3* gene, is a transmembrane serine/threonine kinase that monitors endoplasmic reticulum (ER) stress. Under ER stress caused by unfolded/misfolded protein accumulation, PERK undergoes autophosphorylation at specific residues, including Thr982. leading to its activation. This phosphorylation event triggers downstream signaling, primarily through phosphorylation of eukaryotic initiation factor 2α (eIF2α), which transiently inhibits global protein translation while promoting selective stress-response gene expression.

The Phospho-PERK (Thr982) antibody specifically detects PERK phosphorylated at this residue, serving as a marker for ER stress activation. Researchers use it in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate PERK's role in diseases linked to ER dysfunction, including neurodegenerative disorders (e.g., Alzheimer’s), diabetes, cancer, and inflammatory conditions. Its specificity helps differentiate PERK activation from other UPR branches (IRE1α, ATF6) and assess therapeutic targeting of this pathway. Validation typically includes testing in PERK-knockout models or phosphatase-treated samples to confirm signal specificity.