Phospho-JNK (Thr183/Tyr185)抗体;Phospho-JNK (Thr183/Tyr185) Antibody—艾普蒂 新品

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     Western blot analysis of Phospho-JNK (Thr183/Tyr185) in various lysates using Phospho-JNK (Thr183/Tyr185) antibody.    

  
  

Phospho-JNK (Thr183/Tyr185) antibodies are essential tools for studying the activation status of c-Jun N-terminal kinase (JNK), a member of the mitogen-activated protein kinase (MAPK) family. JNKs, including isoforms JNK1. JNK2. and JNK3. are activated in response to cellular stressors (e.g., UV radiation, cytokines, oxidative stress) and play critical roles in apoptosis, inflammation, and differentiation. Their activation requires dual phosphorylation at conserved Thr183 and Tyr185 residues within the activation loop. Antibodies targeting these phosphorylated sites specifically detect the active, phosphorylated form of JNK, enabling researchers to investigate JNK signaling dynamics in various contexts.

These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and flow cytometry to assess JNK activation in cell lysates, tissue samples, or experimental models. Their applications span cancer research (e.g., studying pro-apoptotic signaling), neurobiology (e.g., neuronal stress responses), and immunology (e.g., inflammatory pathways). Validation often includes testing knockout controls or phosphatase-treated samples to confirm specificity. As dysregulated JNK activity is implicated in diseases like Alzheimer’s, cancer, and autoimmune disorders, Phospho-JNK antibodies are vital for both mechanistic studies and therapeutic target validation.