Phospho-EDG1 (Thr236)抗体;Phospho-EDG1 (Thr236) Antibody—艾普蒂 新品

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Western blot analysis using RAB4A mouse mAb against Jurkat (1), HeLa (2), A549 (3), HEK293 (4), K562 (5), NIH3T3 (6), PC-12 (7), and COS7 (8) cell lysate.    

  
  

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     Flow cytometric analysis of MCF-7 cells using RAB4A mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using RAB4A mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using RAB4A mouse mAb with DAB staining.    

  
  

The Phospho-EDG1 (Thr236) antibody is designed to specifically detect EDG1 (endothelial differentiation gene 1), also known as S1PR1 (sphingosine-1-phosphate receptor 1), when phosphorylated at threonine 236. EDG1 is a G protein-coupled receptor (GPCR) that binds sphingosine-1-phosphate (S1P), a bioactive lipid regulating diverse cellular processes, including cell migration, angiogenesis, immune response, and vascular development. Phosphorylation of Thr236. located within the intracellular C-terminal domain of EDG1. is a critical post-translational modification associated with receptor desensitization and internalization following S1P activation. This event is often mediated by GPCR kinases (GRKs), which attenuate downstream signaling pathways such as ERK, Akt, and Rho GTPase cascades.

The antibody serves as a tool to study EDG1 activation dynamics in response to physiological or pathological stimuli, including growth factors, cytokines, or hypoxia. It is widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess receptor phosphorylation status in cell lines, tissues, or disease models. Dysregulation of EDG1/S1P signaling has been implicated in cancer progression, inflammatory disorders, and vascular diseases, making this antibody valuable for investigating molecular mechanisms in these contexts. Researchers often validate its specificity using knockout controls or phosphatase-treated samples. Species reactivity typically includes human, mouse, and rat, supporting cross-species translational studies.