Phospho-PTEN (Ser380)抗体;Phospho-PTEN (Ser380) Antibody—艾普蒂

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Flow cytometric analysis of HeLa cells using Rab3a mouse mAb (green) and negative control (red).    

  
  

The Phospho-PTEN (Ser380) antibody is a critical tool for studying post-translational modifications of the tumor suppressor protein PTEN (Phosphatase and Tensin Homolog). PTEN acts as a lipid phosphatase that counteracts the PI3K/AKT/mTOR signaling pathway by dephosphorylating phosphatidylinositol (3.4.5)-trisphosphate (PIP3), thereby regulating cell growth, survival, and metabolism. Its activity and stability are tightly regulated by phosphorylation, particularly within its C-terminal regulatory domain. Serine 380 (Ser380) is a key phosphorylation site in this region, along with neighboring residues (e.g., Ser382. Thr383). Phosphorylation at Ser380. mediated by kinases such as CK2 (Casein Kinase 2), induces a conformational change that stabilizes PTEN but reduces its membrane affinity and phosphatase activity. This modification creates a "closed" state, limiting PTEN’s tumor-suppressive function while protecting it from proteasomal degradation.

The Phospho-PTEN (Ser380) antibody specifically detects PTEN phosphorylated at Ser380. enabling researchers to assess PTEN regulation in cellular contexts. It is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study PTEN inactivation mechanisms in cancer, neurodegeneration, and metabolic disorders. Dysregulated PTEN phosphorylation is linked to tumor progression, as hyperphosphorylation may impair its tumor-suppressive role. This antibody helps elucidate how PTEN activity is modulated by signaling pathways and informs therapeutic strategies targeting PTEN-related pathologies. Validation in specific experimental models is essential, as phosphorylation patterns may vary across cell types or disease states.