Phospho-ATF4 (Ser219)抗体;Phospho-ATF4 (Ser219) Antibody—艾普蒂

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     Western blot analysis of Phospho-ATF4 (Ser219) in COLO lysates using Phospho-ATF4 (Ser219) antibody.    

  
  

The Phospho-ATF4 (Ser219) antibody is a specialized tool used to detect the activated form of Activating Transcription Factor 4 (ATF4), a key regulator of cellular stress responses. ATF4 is a basic leucine zipper (bZIP) transcription factor that integrates signals from various stress pathways, including the integrated stress response (ISR), endoplasmic reticulum (ER) stress, and amino acid deprivation. Its phosphorylation at serine 219 (Ser219) is critical for modulating its transcriptional activity, stability, or interaction with co-regulators. This post-translational modification often occurs downstream of kinases such as PERK (PKR-like ER kinase) or RSK (ribosomal S6 kinase), linking cellular stress signals to adaptive gene expression programs.

The Phospho-ATF4 (Ser219) antibody specifically recognizes this phosphorylated epitope, enabling researchers to study ATF4 activation dynamics in conditions like oxidative stress, nutrient deprivation, or ER dysfunction. It is widely used in Western blotting, immunofluorescence, and immunoprecipitation to investigate ATF4's role in processes such as autophagy, apoptosis, and metabolic adaptation. Dysregulation of ATF4 phosphorylation has been implicated in cancer, neurodegenerative diseases, and metabolic disorders, making this antibody valuable for both mechanistic studies and translational research. Validation typically includes testing in knockout models or phosphorylation-deficient mutants to ensure specificity.