Phospho-Sp1 (Thr739)抗体;Phospho-Sp1 (Thr739) Antibody—艾普蒂 新品

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     Western blot analysis of Phospho-Sp1 (Thr739) in COS7 lysates using Phospho-Sp1 (Thr739) antibody.    

  
  

The Phospho-Sp1 (Thr739) antibody is a specialized tool used to detect the phosphorylation status of Sp1 (Specificity Protein 1) at threonine residue 739. a post-translational modification critical for regulating Sp1's transcriptional activity. Sp1 is a ubiquitously expressed transcription factor belonging to the Sp/KLF family, playing key roles in basal and inducible gene expression, particularly for genes involved in cell growth, differentiation, and apoptosis. Phosphorylation at Thr739 has been linked to functional modulation of Sp1. influencing its DNA-binding capacity, protein-protein interactions, and stability. This modification is often mediated by kinases such as ERK or CDKs in response to cellular signals like growth factors, stress, or oncogenic stimuli, thereby connecting Sp1 activity to pathways like MAPK signaling or cell cycle progression.

The antibody is widely utilized in research to study Sp1's role in physiological and pathological contexts, including cancer, neurodegeneration, and metabolic disorders. For instance, hyperphosphorylation of Sp1 at Thr739 has been observed in certain cancers, correlating with enhanced tumor proliferation or chemoresistance. Validated for applications like Western blotting, immunoprecipitation, and immunofluorescence, this antibody aids in elucidating Sp1's regulatory mechanisms under varying cellular conditions. Specificity is typically confirmed using phosphorylation-blocking peptides or Sp1-deficient cell lines. By enabling precise detection of Thr739 phosphorylation, this antibody provides insights into how Sp1 integrates extracellular signals into transcriptional programs, making it a valuable tool for dissecting gene regulation networks and potential therapeutic targets.