Phospho-STAT3 (Tyr705)抗体;Phospho-STAT3 (Tyr705) Antibody—艾普蒂

+ +

•  

   

     Western blot analysis of Phospho-STAT3 (Tyr705) in various lysates using Phospho-STAT3 (Tyr705) antibody.    

  
  

The Phospho-STAT3 (Tyr705) antibody is a critical tool for studying the activation status of Signal Transducer and Activator of Transcription 3 (STAT3), a transcription factor involved in cell proliferation, survival, and immune responses. STAT3 is activated via tyrosine phosphorylation, primarily at residue Tyr705. which occurs in response to cytokines, growth factors (e.g., IL-6. EGF), or oncogenic signals. Upon phosphorylation, STAT3 dimerizes, translocates to the nucleus, and regulates genes linked to inflammation, cancer progression, and apoptosis. Dysregulated STAT3 activity, particularly hyperphosphorylation at Tyr705. is associated with numerous cancers, autoimmune disorders, and inflammatory diseases, making this modification a key biomarker for pathological states.

Phospho-STAT3 (Tyr705) antibodies are widely used in techniques like Western blotting, immunohistochemistry, and flow cytometry to detect activated STAT3 in cell lysates, tissues, or clinical samples. These antibodies specifically recognize the phosphorylated Tyr705 epitope, distinguishing active STAT3 from its inactive form. Researchers employ them to investigate signaling pathways (e.g., JAK-STAT), evaluate therapeutic inhibitors targeting STAT3 activation, or correlate phospho-STAT3 levels with disease outcomes. Validation of these antibodies is crucial, as off-target binding or cross-reactivity with other phosphorylated STAT family members may lead to inaccurate interpretations. Overall, this antibody is indispensable for mechanistic studies and translational research in oncology, immunology, and drug development.