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网站主页 化工产品目录 生物化工 抗体 染色质/表观遗传/细胞周期 G2M/DNA Damage Checkpoint PHOSPHO-P53(SER15)抗体 Phospho-p53 (Ser15)抗体
  • Phospho-p53 (Ser15)抗体;Phospho-p53 (Ser15) Antibody—艾普蒂
  • Phospho-p53 (Ser15)抗体;Phospho-p53 (Ser15) Antibody—艾普蒂
  • Phospho-p53 (Ser15)抗体;Phospho-p53 (Ser15) Antibody—艾普蒂

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Phospho-p53 (Ser15)抗体;Phospho-p53 (Ser15) Antibody—艾普蒂

Rabbit Polyclonal Phospho-p53(Ser15) Antibody
询价 20μl 起订
50μl 起订
100μl 起订
上海 更新日期：2025-06-03

上海切尔齐生物科技有限公司

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联系人：周经理
电话：027-19371278702拨打
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邮箱：nuodina88@163.com

产品详情:

中文名称：
Phospho-p53 (Ser15)抗体
英文名称：
Rabbit Polyclonal Phospho-p53(Ser15) Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 9049 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Rabbit
偶联物：
无
靶点：
Phospho-p53 (Ser15)

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF1/20 Human,Mouse,Rat
IHC1/50-1/100 Human,Mouse,Rat
ICC1/50-1/200 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesTP53; P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
Entrez GeneID7157
WB Predicted band sizeCalculated MW: 44 kDa; Observed MW: 48 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Rat
ImmunogenThe antiserum was produced against synthesized peptide derived from human p53 around the phosphorylation site of Ser15. AA range:1-50
FormulationPurified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

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       Western blot analysis of Phospho-p53 (Ser15) in HeLa treated with HU lysates using Phospho-p53 (Ser15) antibody.The lane on the right is blocked with the Phospho- peptide.    


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       Western blot analysis of Phospho-p53 (Ser15) in untreated and Phospho-p53 (Ser15) treated with HU in 293T lysates using Phospho-p53 (Ser15) antibody    


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       Immunofluorescence analysis of Phospho-p53 (Ser15) in rat spleen tissue using antibody.    


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       Immunohistochemistry analysis of paraffin-embedded rat spleen tissue using Phospho-p53 (Ser15) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.    


           

参考文献

以下是关于Phospho-p53 (Ser15)抗体的3-4篇参考文献及其简要概括：

1. **文献名称**：*Activation of the ATM kinase by ionizing radiation and phosphorylation of p53*

**作者**：Canman, C.E. et al.

**摘要**：该研究首次揭示了DNA损伤(如电离辐射)后，ATM激酶直接磷酸化p53蛋白的Ser15位点，阻断其与MDM2的相互作用，从而稳定p53并激活其转录活性，促进细胞周期阻滞或凋亡。

2. **文献名称**：*DNA damage-induced phosphorylation of p53 alleviates inhibition by MDM2*

**作者**：Shieh, S.Y. et al.

**摘要**：通过体外激酶实验和细胞模型，证明DNA损伤后Ser15和Ser20的磷酸化是p53功能激活的关键步骤，磷酸化减弱了MDM2介导的p53泛素化降解，增强其在细胞核内的积累及靶基因调控能力。

3. **文献名称**：*A role for ATR in the DNA damage-induced phosphorylation of p53*

**作者**：Tibbetts, R.S. et al.

**摘要**：研究发现，在复制应激(如紫外线辐射)下，ATR激酶而非ATM负责磷酸化p53的Ser15位点，揭示了不同DNA损伤类型通过不同激酶通路调控p53活性的分子机制。

4. **文献名称**：*Site-specific phosphorylation of p53 by checkpoint kinases in response to UV radiation*

**作者**：Ko, L.J. et al.

**摘要**：利用特异性抗体(包括Phospho-p53 Ser15抗体)，验证了UV诱导的DNA损伤通过ATR/Chk1通路触发p53的Ser15磷酸化，并阐明其与下游凋亡信号通路的关联。

**备注**：上述文献为p53磷酸化机制研究的经典论文，适用于抗体特异性验证、DNA损伤应答机制探讨或相关信号通路研究。

       

背景信息

Phospho-p53 (Ser15) antibody is a key tool for studying the activation and regulatory mechanisms of the tumor suppressor protein p53. which plays a central role in maintaining genomic stability and preventing cancer. The p53 protein is activated in response to cellular stress, such as DNA damage, oncogene activation, or hypoxia, and functions primarily by inducing cell cycle arrest, DNA repair, or apoptosis. Post-translational modifications, particularly phosphorylation, tightly regulate its stability and activity. Phosphorylation at serine 15 (Ser15) is one of the earliest and most critical modifications occurring in the N-terminal transactivation domain of p53 following DNA damage. This modification is primarily mediated by the ATM/ATR kinases, which are activated by DNA damage checkpoints.

The Phospho-p53 (Ser15) antibody specifically detects p53 when phosphorylated at Ser15. serving as a marker for p53 activation. Researchers use this antibody in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study DNA damage response pathways, assess p53-dependent transcriptional activity, and explore mechanisms of tumor suppression or chemoresistance in cancer models. Its application is vital in both basic research and preclinical studies, particularly in understanding how p53 dysfunction contributes to tumorigenesis or influences therapeutic outcomes.

       
Phospho-p53 (Ser15)抗;Phospho-p53 (Ser15);Phospho-p53 (Ser15) ;

公司简介

生物技术有限公司

成立日期 (2年)
注册资本 20万人民币
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 抗体,植物生物技术,细胞培养,蛋白组学

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