Phospho-FOXO1/3 (Ser322/S325)抗体;Phospho-FOXO1/3 (Ser322/S325) Antibody—艾普蒂 新品

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     Western blot analysis of Phospho-FOXO1/3 (Ser322/S325) in 3T3 lysates using Phospho-FOXO1/3 (Ser322/S325) antibody.    

  
  

The Phospho-FOXO1/3 (Ser322/Ser325) antibody is a critical tool for studying the regulation and activity of FOXO transcription factors, which play pivotal roles in cellular processes such as apoptosis, cell cycle arrest, oxidative stress resistance, and metabolism. FOXO1 and FOXO3 are members of the Forkhead box O (FOXO) family, whose activity is tightly regulated by post-translational modifications, particularly phosphorylation. The PI3K/Akt signaling pathway is a primary regulator of FOXO proteins; upon activation by growth factors or insulin, Akt phosphorylates FOXO1 at Ser322 and FOXO3 at Ser325. This phosphorylation induces their nuclear exclusion by promoting binding to 14-3-3 proteins, thereby sequestering them in the cytoplasm and inhibiting their transcriptional activity.

The Phospho-FOXO1/3 (Ser322/Ser325) antibody specifically detects this phosphorylation event, serving as a marker for Akt-mediated inactivation of FOXO proteins. Researchers use this antibody in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate cellular responses to stimuli such as growth factors, oxidative stress, or metabolic changes. Dysregulation of FOXO phosphorylation is implicated in diseases including cancer, diabetes, and aging-related disorders, making this antibody valuable for both basic research and therapeutic development. By monitoring FOXO phosphorylation status, scientists can elucidate signaling pathway dynamics and identify potential targets for modulating FOXO-dependent cellular outcomes.