Phospho-MEK2 (Thr394)抗体;Phospho-MEK2 (Thr394) Antibody—艾普蒂 新品

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     Western blot analysis of Phospho-MEK2 (Thr394) in various lysates using Phospho-MEK2 (Thr394) antibody.    

  
  

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     Western blot analysis of Phospho-MEK2 (Thr394) in VEC lysates using Phospho-MEK2 (T394) antibody.    

  
  

The Phospho-MEK2 (Thr394) antibody is a specialized tool used to detect the activated form of MEK2 (MAPK/ERK kinase 2), a critical serine/threonine kinase in the RAS/RAF/MEK/ERK signaling pathway. MEK2 plays a central role in transmitting extracellular signals, such as growth factors and cytokines, to intracellular targets, regulating cell proliferation, differentiation, and survival. Its activity is tightly controlled by phosphorylation at specific residues. Phosphorylation at Thr394. located in the activation loop of MEK2. is associated with its enzymatic activation, often triggered by upstream RAF kinases. This modification facilitates MEK2's ability to phosphorylate its downstream substrate ERK1/2. propagating the signal cascade.

The Phospho-MEK2 (Thr394) antibody is designed to specifically recognize MEK2 when phosphorylated at Thr394. enabling researchers to study MEK2 activation dynamics in response to stimuli or inhibitors. It is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess pathway activation in cancer research, drug development, and cellular signaling studies. Validation typically includes testing in cell lines with known pathway activation (e.g., EGF-stimulated cells) or MEK2 knockout controls to ensure specificity. Dysregulation of MEK2 phosphorylation is implicated in cancers and other diseases, making this antibody valuable for both basic research and therapeutic targeting studies.