Phospho-ATF2 (Thr71)抗体;Phospho-ATF2 (Thr71) Antibody—艾普蒂

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     Western blot analysis of Phospho-ATF2 (T71) in (1) HeLa lysates; (2) HeLa lysates treated with Anisomycin using Phospho-ATF2 (Thr71) antibody.    

  
  

The Phospho-ATF2 (Thr71) antibody is a specialized tool used to detect the activated form of Activating Transcription Factor 2 (ATF2), a member of the leucine zipper family of DNA-binding proteins. ATF2 plays a critical role in cellular stress responses, apoptosis, and proliferation by regulating the transcription of target genes. Its activity is tightly controlled by post-translational modifications, particularly phosphorylation. The Thr71 residue, located within the transactivation domain of ATF2. is phosphorylated by stress-activated kinases such as JNK (c-Jun N-terminal kinase) and p38 MAPK (mitogen-activated protein kinase) in response to DNA damage, cytokines, or oxidative stress. This phosphorylation event enhances ATF2's transcriptional activity by promoting dimerization and DNA binding.

The Phospho-ATF2 (Thr71) antibody specifically recognizes ATF2 when phosphorylated at Thr71. enabling researchers to study its activation status in various experimental models. It is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate signaling pathways involving stress responses, inflammation, or oncogenesis. Dysregulation of ATF2 phosphorylation has been implicated in cancer progression, neurodegenerative diseases, and immune disorders, making this antibody valuable for both mechanistic studies and therapeutic target validation. Its specificity and reliability depend on proper validation, including knockout controls and peptide competition assays.