Phospho-Src (Tyr419)抗体;Phospho-Src (Tyr419) Antibody—艾普蒂

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     Western blot analysis of Phospho-Src (Y419) in A431 lysates treated with pervanadate using Phospho-Src (Tyr419) antibody.    

  
  

Phospho-Src (Tyr419) antibody is a crucial tool for studying the activation status of Src kinase, a non-receptor tyrosine kinase involved in diverse cellular signaling pathways. Src plays a pivotal role in regulating processes such as cell proliferation, differentiation, adhesion, and migration. Its activity is tightly controlled by phosphorylation and dephosphorylation events. The autophosphorylation site Tyr419 (corresponding to Tyr418 in rodents) in the catalytic domain is a key marker of Src activation. Phosphorylation at this site induces a conformational shift that enhances kinase activity, enabling downstream signaling through pathways like MAPK/ERK, PI3K/Akt, and FAK, which are implicated in oncogenesis, metastasis, and cytoskeletal remodeling.

This antibody specifically recognizes Src phosphorylated at Tyr419. allowing researchers to detect active Src in various experimental models. It is widely used in Western blotting, immunohistochemistry, and immunofluorescence to investigate Src activation in cancer, bone metabolism, and neurological disorders. Dysregulated Src activity, marked by elevated Tyr419 phosphorylation, is associated with tumor progression, drug resistance, and invasive phenotypes in cancers such as breast, colon, and pancreatic cancer. Additionally, it serves as a biomarker for studying Src's role in osteoclast function and neurodegenerative diseases. Researchers must validate the antibody for species specificity, as cross-reactivity with other Src-family kinases (e.g., Lyn, Fyn) may occur depending on sequence homology. Its application advances understanding of Src-driven mechanisms and therapeutic targeting in disease contexts.