Phospho-PAK1/2/3 (Ser144/Ser141/Ser154)抗体;Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Antibody—艾普蒂

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     Western blot analysis of Phospho-PAK1/2/3 in HeLa lysates treated with lambda phosphatase using Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) antibody.    

  
  

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     Immunohistochemistry analysis of paraffin-embedded mouse brain using Phospho-PAK1/2/3 (S144+S141+S139) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.    

  
  

The Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) antibody detects the activated forms of p21-activated kinases (PAKs), a family of serine/threonine kinases critical for cytoskeletal remodeling, cell motility, and survival. PAK1 (phosphorylated at Ser144), PAK2 (Ser141), and PAK3 (Ser154) are activated via auto-phosphorylation upon binding to GTPases like Rac or Cdc42. which trigger conformational changes to release the kinase domain from auto-inhibition. This phosphorylation event is a hallmark of PAK activation, driving downstream signaling cascades involving MAPK, PI3K/AKT, and other pathways.

The antibody is widely used in research to study PAK activation dynamics in physiological processes (e.g., neuronal development, immune responses) and pathological conditions, including cancer metastasis, neurodegenerative diseases, and cardiovascular disorders. It is validated for applications such as Western blotting, immunofluorescence, and immunohistochemistry to assess tissue- or cell-specific PAK activation. Researchers often pair it with total PAK antibodies to quantify activation ratios. Specificity is ensured by targeting conserved but distinct phospho-sites across PAK isoforms, though cross-reactivity should be empirically confirmed. Proper sample preparation (e.g., phosphatase inhibitors) is essential to preserve phosphorylation signals. This tool has advanced understanding of PAK-mediated signaling in disease mechanisms and therapeutic targeting.