Phospho-GSK3 (Tyr216/Tyr279)抗体;Phospho-GSK3 (Tyr216/Tyr279) Antibody—艾普蒂

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     Western blot analysis of Phospho-GSK3 (alpha/beta) (Tyr216/Tyr279) in C6, 3T3 lysates using Phospho-GSK3 (Tyr216/Tyr279) antibody.    

  
  

The Phospho-GSK3 (Tyr216/Tyr279) antibody detects glycogen synthase kinase-3 (GSK3) when phosphorylated at specific tyrosine residues critical for its activity. GSK3 exists as two isoforms, GSK3α and GSK3β, which play central roles in cellular signaling pathways, including Wnt, insulin, and growth factor signaling. Unlike its inhibitory phosphorylation at serine residues (Ser21 in GSK3α and Ser9 in GSK3β), tyrosine phosphorylation at Tyr216 (GSK3β) and Tyr279 (GSK3α) enhances kinase activity. These residues reside in the activation loop of the kinase domain, and their phosphorylation stabilizes the active conformation, promoting substrate binding and enzymatic function.

This phosphorylation event is thought to occur via autophosphorylation during GSK3 maturation or through upstream kinases, though regulatory mechanisms remain less defined compared to serine phosphorylation. The Phospho-GSK3 (Tyr216/Tyr279) antibody is widely used in research to study GSK3 activation dynamics in conditions such as metabolic disorders, neurodegenerative diseases (e.g., Alzheimer’s, where GSK3β hyperactivation contributes to tau hyperphosphorylation), and cancer. It is applied in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess GSK3 activity in response to stimuli or therapeutic interventions. Understanding GSK3 regulation via tyrosine phosphorylation provides insights into its dual roles as a tumor suppressor or promoter, depending on context, and informs drug development targeting GSK3 dysregulation.