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网站主页 Phospho-Histone H2A.X (Ser139)抗体 Phospho-Histone H2A.X (Ser139)抗体
  • Phospho-Histone H2A.X (Ser139)抗体;Phospho-Histone H2A.X (Ser139) Antibody—艾普蒂
  • Phospho-Histone H2A.X (Ser139)抗体;Phospho-Histone H2A.X (Ser139) Antibody—艾普蒂
  • Phospho-Histone H2A.X (Ser139)抗体;Phospho-Histone H2A.X (Ser139) Antibody—艾普蒂

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Phospho-Histone H2A.X (Ser139)抗体;Phospho-Histone H2A.X (Ser139) Antibody—艾普蒂

Mouse Monoclonal Phospho-HistoneH2A.X(Ser139) Antibody
询价 20μl 起订
50μl 起订
100μl 起订
上海 更新日期：2025-06-03

上海切尔齐生物科技有限公司

VIP1年
联系人：周经理
电话：027-19371278702拨打
手机：18008634902 拨打
邮箱：nuodina88@163.com

产品详情:

中文名称：
Phospho-Histone H2A.X (Ser139)抗体
英文名称：
Mouse Monoclonal Phospho-HistoneH2A.X(Ser139) Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 9012 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Mouse
偶联物：
无
靶点：
Phospho-Histone H2A.X (Ser139)

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC咨询技术 Human,Mouse,Rat
ICC1/50-1/200 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa咨询技术 Human,Mouse,Rat
   

产品详情

AliasesH2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X; gamma H2A.X
Entrez GeneID3014
clone9D4
WB Predicted band sizeCalculated MW: 15 kDa; Observed MW: 15 kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse
ImmunogenSynthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X.
FormulationPurified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

+ +

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       Western blot analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyureatreated 3T3 lysates using Phospho-Histone H2A.X (Ser139) antibody.    


  •  

     

       Immunofluorescence analysis of Phospho-Histone H2A.X (Ser139) in 3T3 or Hydroxyureatreated 3T3 using Phospho-Histone H2A.X (Ser139) antibody.    


  •  

     

       Immunofluorescence analysis of Phospho-Histone H2A.X (Ser139) in A549(upper, untreated or Hydroxyureatreated) and Hela(lower, untreated or Hydroxyureatreated) using Phospho-Histone H2A.X (Ser139) antibody.    


           

参考文献

1. **"DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139"**

- **作者**: Rogakou, E.P., Pilch, D.R., Orr, A.H., Ivanova, V.S., Bonner, W.M.

- **摘要**: 该研究首次报道了DNA双链断裂(DSBs)后组蛋白H2AX在Ser139位点的磷酸化(γ-H2AX)，揭示了其在DNA损伤应答中的关键作用，并建立了γ-H2AX作为DSBs的分子标记。

2. **"γ-H2AX as a biomarker of DNA damage induced by ionizing radiation in human peripheral blood lymphocytes"**

- **作者**: Sedelnikova, O.A., Rogakou, E.P., Panyutin, I.G., Bonner, W.M.

- **摘要**: 研究通过免疫荧光技术验证了γ-H2AX抗体在检测电离辐射诱导的DNA损伤中的应用，证明γ-H2AX焦点数量与辐射剂量呈正相关，支持其作为辐射暴露的生物标志物。

3. **"The role of γ-H2AX in cancer treatment"**

- **作者**: Bonner, W.M., Redon, C.E., Dickey, J.S., Nakamura, A.J., Sedelnikova, O.A., et al.

- **摘要**: 综述了γ-H2AX在癌症治疗中的重要性，强调其作为化疗和放疗后DNA损伤的动态指标，可用于评估治疗效果及预测患者预后。

4. **"Evidence for a lack of DNA double-strand break repair in human cells exposed to very low X-ray doses"**

- **作者**: Rothkamm, K., Löbrich, M.

- **摘要**: 利用γ-H2AX抗体检测低剂量X射线引起的DNA损伤，发现极低剂量下细胞无法有效修复DSBs，为辐射防护研究提供了关键实验依据。

       

背景信息

Phospho-Histone H2A.X (Ser139) antibody detects the phosphorylated form of histone H2A.X at serine 139. a key biomarker of DNA double-strand breaks (DSBs). Histone H2A.X, a variant of the H2A histone family, becomes rapidly phosphorylated at Ser139 (forming γH2A.X) in response to DNA damage, particularly DSBs induced by ionizing radiation, chemotherapeutic agents, or oxidative stress. This phosphorylation event is mediated by kinases such as ATM, ATR, and DNA-PK, which are activated as part of the DNA damage response (DDR) signaling cascade. The formation of γH2A.X foci serves as a molecular beacon, recruiting repair proteins (e.g., MDC1. 53BP1) to facilitate DNA repair and maintain genomic stability.

The Phospho-Histone H2A.X (Ser139) antibody is widely used in research to visualize and quantify DSBs, assess genomic instability, and study DDR mechanisms. Applications include immunofluorescence, Western blotting, and flow cytometry, enabling insights into cancer biology, radiation therapy, and genotoxic drug development. It is also employed in studying apoptosis, replication stress, and cellular senescence. Validating antibody specificity is critical, as non-specific signals may arise from cross-reactivity or experimental conditions. Overall, this antibody is a cornerstone tool for understanding DNA damage-related pathways and their implications in disease and therapy.

       
Phospho-Histone H2A.;Phospho-Histone H2A.;Phospho-Histone H2A.;

公司简介

生物技术有限公司

成立日期 (2年)
注册资本 20万人民币
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 抗体,植物生物技术,细胞培养,蛋白组学

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