Phospho-Hormone Sensitive Lipase (Ser855)抗体;Phospho-Hormone Sensitive Lipase (Ser855) Antibody—艾普蒂

+ +

•  

   

     Western blot analysis of Hormone sensitive lipase/HSL (Phospho-Ser855) in A549, HL-60, U2OS lysates using Phospho-Hormone Sensitive Lipase (Ser855) antibody.    

  
  

Phospho-Hormone Sensitive Lipase (Ser855) antibody is a specialized tool used to detect the activated form of Hormone-Sensitive Lipase (HSL), a key enzyme regulating lipid metabolism. HSL plays a central role in hydrolyzing stored triglycerides in adipose tissue and cholesterol esters in steroidogenic tissues, releasing free fatty acids and cholesterol for energy production or hormone synthesis. Its activity is tightly controlled by phosphorylation at specific serine residues, including Ser855. which is part of a regulatory mechanism mediated by hormonal signals such as catecholamines (via β-adrenergic receptors) and insulin.

Phosphorylation at Ser855. located in the N-terminal regulatory domain of HSL, is associated with enzyme activation and translocation to lipid droplets. This post-translational modification is primarily triggered by cyclic AMP (cAMP)-dependent protein kinase A (PKA) signaling, which responds to fasting, stress, or exercise. The Phospho-HSL (Ser855) antibody specifically recognizes this phosphorylation event, enabling researchers to study dynamic changes in HSL activation under various physiological or pathological conditions, such as obesity, diabetes, or metabolic syndrome. It is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to explore tissue-specific lipolytic regulation and hormonal crosstalk. Validating antibody specificity through knockout controls or phosphorylation-blocking assays is critical for accurate interpretation of results.