Phospho-beta Arrestin 1 (Ser412)抗体;Phospho-beta Arrestin 1 (Ser412) Antibody—艾普蒂 新品

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     Western blot analysis of Phospho-beta Arrestin 1 (Ser412) in A549, MCF-7, U251, HL-60 lysates using Phospho-beta Arrestin 1 (Ser412) antibody.    

  
  

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     Immunohistochemistry analysis of paraffin-embedded Human lung cancer using beta Arrestin 1 (Phospho-Ser412) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.    

  
  

**Phospho-beta Arrestin 1 (Ser412) Antibody Background**

Beta-arrestin 1 (ARRB1) is a multifunctional adaptor protein critical in regulating G protein-coupled receptor (GPCR) signaling. Upon receptor activation, beta-arrestin 1 is recruited to phosphorylated GPCRs, facilitating receptor desensitization, internalization, and initiation of downstream signaling pathways. Phosphorylation at Ser412 is a key post-translational modification that modulates beta-arrestin 1’s conformational state, influencing its interactions with receptors, clathrin, and other signaling effectors. This phosphorylation event, often mediated by kinases such as ERK1/2. enhances beta-arrestin’s scaffolding functions, promoting MAPK pathway activation or receptor trafficking.

The Phospho-beta Arrestin 1 (Ser412) antibody specifically detects beta-arrestin 1 when phosphorylated at Ser412. serving as a vital tool to study GPCR signaling dynamics, receptor internalization, and arrestin-dependent signaling cascades. Researchers use this antibody in techniques like Western blotting, immunofluorescence, or immunoprecipitation to investigate phosphorylation-dependent mechanisms in cellular contexts, including immune regulation, cancer progression, and neurological disorders. Its application helps elucidate how beta-arrestin 1’s phosphorylation status fine-tunes cellular responses to extracellular stimuli, offering insights into therapeutic targets for diseases involving dysregulated GPCR signaling. Validation of the antibody includes testing specificity via knockout controls or phosphopeptide competition assays.