Phospho-PRC1 (Thr481)抗体;Phospho-PRC1 (Thr481) Antibody—艾普蒂

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     Western blot analysis of Phospho-PRC1 (Thr481) in Hela, Jurkat lysates using Phospho-PRC1 (Thr481) antibody.    

  
  

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     Immunocytochemistry analysis of Phospho-PRC1 (Thr481) (green) in Hela using Phospho-PRC1 (Thr481) antibody,and DAPI(blue)    

  
  

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     Immunohistochemistry analysis of paraffin-embedded Human lung cancer using PRC1 (Phospho-Thr481) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.    

  
  

The Phospho-PRC1 (Thr481) antibody is a specialized tool used to detect the phosphorylation of PRC1 (Polycomb Repressive Complex 1) at threonine residue 481. PRC1. a key component of the Polycomb group (PcG) protein family, plays a critical role in epigenetic gene silencing by mediating monoubiquitination of histone H2A at lysine 119 (H2AK119ub). This activity facilitates chromatin compaction and transcriptional repression, working in concert with PRC2 (which methylates histone H3 at lysine 27) to maintain cellular memory and regulate developmental gene expression programs.

Phosphorylation of PRC1 at Thr481 is associated with its functional regulation during the cell cycle, particularly in mitosis. Studies suggest this post-translational modification may influence PRC1’s interaction with other proteins or its subcellular localization, impacting its role in chromosome condensation, spindle assembly, or cytokinesis. The phosphorylation event is likely mediated by cyclin-dependent kinases (CDKs) or mitotic kinases, linking PRC1 activity to cell cycle progression.

The Phospho-PRC1 (Thr481) antibody is widely used in research to explore PRC1’s dual roles in epigenetic regulation and mitotic processes. It aids in studying dysregulation of Polycomb signaling in cancers, developmental disorders, and other diseases. Validated in applications like Western blotting and immunofluorescence, this antibody helps elucidate mechanisms underlying PRC1 phosphorylation and its implications for genome stability, cell division, and disease pathogenesis.