Acetyl-Histone H3 (Lys14)抗体;Acetyl-Histone H3 (Lys14) Antibody—艾普蒂

+ +

•  

   

     Western blot analysis of Acetyl-Histone H3 (K14) in C6 lysates using Acetyl-Histone H3 (Lys14) antibody.    

  
  

Acetyl-Histone H3 (Lys14) antibodies are essential tools for studying epigenetic regulation and chromatin dynamics. Histone H3 is a core component of nucleosomes, and post-translational modifications, such as acetylation at specific lysine residues, play a critical role in modulating gene expression. Lysine 14 (K14) acetylation on histone H3 is associated with open chromatin structures and transcriptional activation, particularly in promoter regions of actively transcribed genes. This modification neutralizes the positive charge of lysine, reducing histone-DNA interactions and facilitating access for transcriptional machinery.

These antibodies are widely used in chromatin immunoprecipitation (ChIP), immunofluorescence, and Western blotting to investigate the relationship between histone acetylation, gene regulation, and cellular processes like differentiation, DNA repair, and apoptosis. Dysregulation of H3K14 acetylation has been implicated in cancer, neurodegenerative diseases, and developmental disorders, making these antibodies valuable for both basic research and clinical studies.

Specificity is a key feature; reliable Acetyl-Histone H3 (Lys14) antibodies are rigorously validated to distinguish acetylated K14 from other modified residues (e.g., K9 or K27) or unmodified H3. They enable researchers to map acetylation patterns across genomes, study histone-modifying enzymes (e.g., histone acetyltransferases and deacetylases), and evaluate responses to epigenetic therapies. Proper experimental controls, including peptide competition assays and knockout/knockdown models, are recommended to confirm target specificity in diverse biological contexts.