Acetyl-Histone H4 (Lys5)抗体;Acetyl-Histone H4 (Lys5) Antibody—艾普蒂 新品

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     Western blot analysis of Acetyl-Histone H4 (Lys5) in 3T3 lysates using Acetyl-Histone H4 (Lys5) antibody.Observed band size:11kDa.    

  
  

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     Western blot analysis of Acetyl-Histone H4 (Lys5) in A549, HL-60, U2OS, U87-MG lysates using Acetyl-Histone H4 (Lys5) antibody    

  
  

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     Immunocytochemistry analysis of Acetyl-Histone H4 (Lys5) (green) in Hela using Acetyl-Histone H4 (Lys5) antibody,and DAPI(blue).    

  
  

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     Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Acetyl-Histone H4 (Lys5) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.    

  
  

Acetyl-Histone H4 (Lys5) antibodies are essential tools for studying epigenetic regulation linked to histone acetylation, a post-translational modification critical for chromatin structure and gene expression. Histone H4 acetylation at lysine 5 (H4K5ac) is associated with relaxed chromatin states, facilitating transcriptional activation by promoting accessibility of DNA to transcription factors and RNA polymerase. This modification is dynamically regulated by histone acetyltransferases (HATs) and histone deacetylases (HDACs), which balance acetylation levels to control cellular processes like DNA replication, repair, and differentiation.

H4K5ac often cooperates with other acetylated lysine residues (e.g., H4K8. H4K12. H4K16) to establish specific acetylation patterns that influence nucleosome stability and transcriptional activity. Studies suggest H4K5ac is enriched at active gene promoters and enhancers, serving as a marker for regulatory regions. Dysregulation of H4K5ac has been implicated in diseases such as cancer, neurodegeneration, and metabolic disorders, making its detection crucial for understanding disease mechanisms.

These antibodies are widely used in techniques like chromatin immunoprecipitation (ChIP), Western blotting, and immunofluorescence to map acetylation sites, assess epigenetic states, and evaluate drug effects targeting HDACs or HATs. Validated for specificity, they help researchers explore how H4K5ac contributes to cellular identity, stress responses, and therapeutic interventions in epigenetic diseases.