DENN抗体;DENN Antibody—艾普蒂 新品

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     Western blot analysis of DENN expression in HeLa cell lysate.    

  
  

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

DENN (Differentially Expressed in Normal and Neoplastic cells) antibodies are essential tools for studying the DENN domain-containing proteins, which play critical roles in intracellular membrane trafficking and signal transduction. The DENN domain, first identified in the 1990s, is a conserved structural module found in guanine nucleotide exchange factors (GEFs) that activate Rab GTPases—key regulators of vesicle transport, organelle dynamics, and autophagy. Over 20 DENN domain-containing proteins (e.g., DENND1-4. MADD, AEX-3) have been identified in humans, each with distinct cellular functions.

DENN antibodies are widely used to investigate the localization, expression, and interactions of these proteins under physiological or pathological conditions. Their development has facilitated discoveries linking DENN proteins to diseases, including cancer, neurodevelopmental disorders (e.g., schizophrenia), and autoimmune conditions. For instance, DENND1A variants are associated with polycystic ovary syndrome, while DENND2B mutations correlate with intellectual disability. Researchers employ DENN antibodies in techniques like Western blotting, immunofluorescence, and co-immunoprecipitation to dissect molecular mechanisms, such as Rab activation pathways or synaptic vesicle regulation. Challenges remain in isoform specificity due to structural similarities among DENN family members, driving ongoing efforts to refine antibody design. These reagents continue to advance our understanding of membrane trafficking networks and their therapeutic potential.