Glycerolkinase抗体;Glycerolkinase Antibody—艾普蒂 新品

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     Western blot analysis of Glycerol kinase expression in HepG2 cell lysate.    

  
  

Glycerol kinase (GK) is a metabolic enzyme that catalyzes the ATP-dependent phosphorylation of glycerol to glycerol-3-phosphate, a critical substrate for glycolysis and lipid biosynthesis. GK plays a key role in energy homeostasis, linking lipid and carbohydrate metabolism. Deficiencies in GK activity are associated with hyperglycerolemia and glycerol kinase deficiency (GKD), a rare X-linked disorder characterized by metabolic crises and neurological symptoms. GK also interacts with hormonal signaling pathways, making it relevant to studies on diabetes, obesity, and metabolic syndromes.

Antibodies targeting glycerol kinase are essential tools for investigating its expression, localization, and function in cellular and tissue contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess GK levels in research models, including genetically modified organisms or disease-specific cell lines. Polyclonal and monoclonal variants are available, often raised against recombinant GK proteins or specific epitopes. Species-specific reactivity (e.g., human, mouse, rat) is a key consideration for experimental design. Additionally, GK antibodies aid in studying metabolic adaptations in conditions like fasting, insulin resistance, or cancer, where glycerol metabolism is dysregulated. Validation via knockout controls or enzymatic assays is critical to ensure specificity, given structural similarities among kinase family members. These antibodies contribute to both basic research and potential diagnostic applications.