HTSF1抗体;HTSF1 Antibody—艾普蒂 新品

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     Western blot analysis of HTSF1 expression in Jurkat cell lysate.    

  
  

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

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     Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution)    

  
  

The HTSF1 antibody is a immunological tool designed to detect and study Heat Shock Transcription Factor 1 (HSF1), a critical regulator of cellular stress responses. HSF1. encoded by the HSF1 gene in humans, orchestrates the heat shock response by binding to heat shock elements (HSEs) in DNA, thereby activating the transcription of molecular chaperones like HSP70 and HSP90. These chaperones assist in protein folding, mitigate proteotoxic stress, and promote cell survival under conditions such as heat, oxidative stress, or chemical exposure. Dysregulation of HSF1 is implicated in cancer, neurodegenerative diseases, and aging.

The HTSF1 antibody, typically developed in rabbit or mouse models, enables the detection of HSF1 protein levels, localization, and activation status (e.g., phosphorylation) via techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). Researchers utilize this antibody to investigate HSF1's role in stress adaptation, tumor progression (where HSF1 supports oncogenesis by stabilizing proteostasis), and its crosstalk with signaling pathways like mTOR and NF-κB. Commercial variants often undergo validation in knockout cell lines to ensure specificity. Its applications span basic molecular biology, drug discovery (targeting HSF1 for cancer therapy), and studies on age-related protein aggregation disorders.