Kir2.1抗体;Kir2.1 Antibody—艾普蒂 新品

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     Western blot analysis of Kir2.1 expression in A549 cell lysate.    

  
  

Kir2.1 antibodies are immunological tools designed to detect and study Kir2.1 proteins, a subset of inwardly rectifying potassium (Kir) channels critical for maintaining resting membrane potential and regulating cellular excitability. Encoded by the *KCNJ2* gene, Kir2.1 channels are widely expressed in excitable tissues, including cardiac muscle, skeletal muscle, and neurons. These channels facilitate potassium influx, stabilizing membrane potential and shaping action potential duration. Dysfunction of Kir2.1 is linked to human diseases such as Andersen-Tawil syndrome (a rare disorder causing cardiac arrhythmias, muscle weakness, and developmental abnormalities) and certain forms of cardiac arrhythmia.

Kir2.1 antibodies are typically produced in hosts like rabbits or mice, targeting specific epitopes within the protein’s intracellular or extracellular domains. They are employed in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression, localization, and post-translational modifications. Validation of these antibodies often involves knockout cell lines or tissues to confirm specificity.

Research applications include investigating Kir2.1’s role in cardiac electrophysiology, neuronal signaling, and disease mechanisms. For example, studies using these antibodies have elucidated how Kir2.1 loss contributes to arrhythmia susceptibility or altered neuronal excitability. However, variability in antibody performance (e.g., cross-reactivity with other Kir isoforms) necessitates careful experimental controls. Commercial and custom Kir2.1 antibodies remain indispensable for advancing understanding of potassium channel biology and developing targeted therapies for channelopathies.