Phospho-CaMKIIalpha(T286)抗体;Phospho-CaMKIIalpha(T286) Antibody—艾普蒂 新品

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     Western blot analysis of Phospho-CaMKII alpha (T286) expression in Mouse brain treated with Lambda phosphatase cell lysate.    

  
  

The Phospho-CaMKIIα (Thr286) antibody detects the activated form of calcium/calmodulin-dependent protein kinase II alpha (CaMKIIα), a key regulator of synaptic plasticity and memory formation. CaMKIIα is a serine/threonine kinase highly enriched in the brain, particularly in postsynaptic densities. Its activation requires calcium/calmodulin binding, which induces autophosphorylation at Thr286—a critical step that sustains kinase activity even after calcium levels decline, enabling long-term potentiation (LTP). This antibody specifically recognizes CaMKIIα phosphorylated at Thr286. making it a vital tool for studying neuronal signaling dynamics, learning/memory mechanisms, and pathologies like Alzheimer’s disease or epilepsy.

Researchers use this antibody in techniques like Western blotting, immunohistochemistry, or immunofluorescence to map spatial-temporal activation patterns in brain tissues or cultured neurons under stimuli (e.g., glutamate, KCl depolarization). It helps differentiate basal vs. activity-dependent phosphorylation states, aiding investigations into synaptic strengthening, dendritic spine remodeling, or drug effects on kinase pathways. Specificity is often confirmed via knockout controls or peptide blocking assays. Notably, Thr286 autophosphorylation also promotes CaMKIIα translocation to synapses, influencing downstream targets (e.g., glutamate receptors). Dysregulation of this pathway is linked to neuropsychiatric disorders, underscoring the antibody’s relevance in both basic and translational neuroscience.