HistoneH3(acetylK27)抗体;HistoneH3(acetylK27) Antibody—艾普蒂

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

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     Western blot analysis of Histone H3 (acetyl K27) expression in HeLa cell treated with TSA cell lysate.    

  
  

Histone H3 acetylated at lysine 27 (H3K27ac) is a post-translational modification associated with active gene regulatory elements, particularly enhancers and promoters. Acetylation of histone H3 at K27 neutralizes the positive charge of lysine residues, reducing their interaction with negatively charged DNA and promoting a more open chromatin structure. This epigenetic mark is strongly linked to transcriptional activation, as it facilitates the recruitment of chromatin-remodeling complexes and transcriptional machinery. H3K27ac is often used as a biomarker to distinguish active enhancers from poised or repressed regulatory regions in genomic studies.

Antibodies targeting H3K27ac are essential tools in chromatin immunoprecipitation (ChIP) assays, including ChIP-seq, to map regulatory elements and study gene expression dynamics. They are also employed in techniques like immunofluorescence, Western blotting, and flow cytometry to assess epigenetic states in various biological contexts. Dysregulation of H3K27ac has been implicated in diseases such as cancer, neurodegenerative disorders, and developmental abnormalities, making these antibodies valuable for both basic research and clinical investigations. Their specificity is critical, as cross-reactivity with other acetylated histone residues (e.g., H3K9ac or H3K14ac) could compromise data interpretation. Validation using knockout models or peptide competition assays is recommended to ensure antibody reliability.