PCYT1A抗体;PCYT1A Antibody—艾普蒂

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     Western blot analysis of PCYT1A expression in A549 cell lysate.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

**Background of PCYT1A Antibody**

PCYT1A (Phosphocholine Cytidylyltransferase 1A) is a key enzyme in phospholipid metabolism, catalyzing the rate-limiting step in the synthesis of phosphatidylcholine (PC), a major component of cell membranes. Encoded by the *PCYT1A* gene, this enzyme localizes to the endoplasmic reticulum and regulates the conversion of phosphocholine to cytidine diphosphate-choline (CDP-choline), a critical precursor for PC biosynthesis. Dysregulation of PCYT1A is linked to metabolic disorders, congenital myopathies, and cancer progression.

Antibodies targeting PCYT1A are essential tools for studying its expression, localization, and functional roles in cellular processes. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate tissue-specific expression patterns or alterations in disease models. PCYT1A antibodies are often validated using knockout cell lines or tissues to confirm specificity.

Research applications include exploring PCYT1A’s involvement in lipid homeostasis, skeletal muscle development, and cancer cell survival. Mutations in *PCYT1A* are associated with rare genetic disorders, such as autosomal recessive limb-girdle muscular dystrophy, making these antibodies valuable for diagnostic and mechanistic studies. Commercial PCYT1A antibodies are typically raised in rabbit or mouse hosts, with reactivity confirmed across human, mouse, and rat samples. Challenges in detection may arise due to low protein abundance, requiring optimized protocols for sensitive assays.