Phospho-RIP3(S232)抗体;Phospho-RIP3(S232) Antibody—艾普蒂 新品

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     Western blot analysis of Phospho-RIP3 (S232) expression in L929 cell lysate.    

  
  

Phospho-RIP3(S232) antibody is a specialized tool used to study the activation of Receptor-Interacting Protein Kinase 3 (RIP3/RIPK3), a key regulator of necroptosis, a form of programmed cell death associated with inflammation and disease. RIP3. a serine/threonine kinase, is activated via phosphorylation within its activation loop, particularly at residues S227. T231. and S232 in humans (corresponding to S232 in some species). Phosphorylation at these sites, often mediated by RIP1 or other upstream signals, induces conformational changes that stabilize RIP3 kinase activity, enabling its role in forming the necrosome complex. This complex triggers downstream phosphorylation of mixed-lineage kinase domain-like protein (MLKL), leading to plasma membrane rupture and inflammatory signaling.

The Phospho-RIP3(S232) antibody specifically recognizes RIP3 phosphorylated at serine 232. serving as a critical marker for detecting necroptosis activation in experimental models. Researchers use this antibody in techniques like Western blotting, immunofluorescence, or immunohistochemistry to investigate RIP3-dependent pathways in conditions such as ischemic injury, neurodegenerative diseases, viral infections, or cancer. Its specificity helps distinguish necroptosis from apoptosis, as caspase-independent cell death relies on RIP3-MLKL signaling. However, interpretation requires caution, as phosphorylation dynamics may vary across cell types or stimuli. Validation using RIP3-deficient cells or kinase inhibitors is recommended to confirm signal specificity. This antibody thus advances understanding of cell death mechanisms and their therapeutic targeting.