HistoneH3(dimethylK4)抗体;HistoneH3(dimethylK4) Antibody—艾普蒂 新品

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     Western blot analysis of Histone H3 (di methyl K4) expression in HeLa cell lysate.    

  
  

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

The Histone H3 (dimethyl K4) antibody is a key tool for studying epigenetic regulation, specifically targeting the dimethylation of lysine 4 on histone H3 (H3K4me2). This post-translational modification is enriched in promoter regions of actively transcribed genes and is associated with transcriptional activation. H3K4me2 is deposited by methyltransferases such as SET1/COMPASS complexes and removed by demethylases like LSD1/KDM1A or JARID1/KDM5 family proteins. It plays a critical role in chromatin remodeling, maintaining an open chromatin state to facilitate transcription factor binding and RNA polymerase II recruitment.

Researchers use this antibody in techniques like chromatin immunoprecipitation (ChIP-seq), immunofluorescence (IF), and Western blotting (WB) to map H3K4me2 distribution and investigate its involvement in cellular processes, including differentiation, development, and disease. Aberrant H3K4me2 levels have been linked to cancers, neurological disorders, and metabolic syndromes, making the antibody valuable for both basic research and clinical studies.

Specificity validation is essential, as cross-reactivity with mono- or trimethylated H3K4 or other histone modifications can occur. Proper controls (e.g., knockout cells or peptide competition assays) are required to confirm antibody reliability. Commercial antibodies often provide validation data across multiple applications, but batch variability may affect performance. Understanding H3K4me2 dynamics through this antibody aids in deciphering gene regulatory mechanisms and identifying therapeutic targets in epigenetic diseases.