CardiacTroponinC抗体;CardiacTroponinC Antibody—艾普蒂 新品

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     Western blot analysis of Cardiac Troponin C expression in Human fetal heart lysate.    

  
  

Cardiac Troponin C (cTnC) is a calcium-binding regulatory subunit of the troponin complex, which plays a central role in mediating calcium-dependent contraction in cardiac muscle. The troponin complex consists of three subunits: Troponin C (TnC), Troponin I (TnI), and Troponin T (TnT). Among these, cTnC is encoded by the *TNNC1* gene and is expressed specifically in cardiac tissue, distinguishing it from its skeletal muscle isoforms (slow-twitch and fast-twitch TnC). This structural and functional specificity makes cTnC a valuable biomarker for cardiac-related studies and diagnostics.

Antibodies targeting cTnC are critical tools in both research and clinical applications. In research, they are used to detect and quantify cTnC expression in cardiac tissues or cell models, aiding studies on myocardial physiology, heart failure, or genetic cardiomyopathies. These antibodies are also employed in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence to visualize spatial and temporal distribution of cTnC. Clinically, while cTnI and cTnT are more commonly used as blood biomarkers for myocardial injury (e.g., myocardial infarction), cTnC-specific antibodies contribute to understanding tissue-specific damage and developing advanced diagnostic assays.

Generation of cTnC antibodies typically involves immunizing host animals with purified recombinant cTnC protein or synthetic peptides derived from its unique amino acid sequences. Monoclonal antibodies offer high specificity, whereas polyclonal antibodies may detect broader epitopes. Validation includes cross-reactivity checks against skeletal muscle TnC isoforms to ensure cardiac specificity. Recent advancements in antibody engineering, such as recombinant or humanized formats, enhance their utility in precision diagnostics and therapeutic monitoring. Overall, cTnC antibodies remain indispensable for unraveling cardiac pathophysiology and improving cardiovascular disease management.