LIMK1抗体;LIMK1 Antibody—艾普蒂

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     Western blot analysis of LIM Kinase 1  expression in U-87MG cell lysate.    

  
  

LIMK1 (LIM domain kinase 1) is a serine/threonine kinase belonging to the LIM kinase family, characterized by the presence of two LIM domains and a kinase domain. It plays a critical role in regulating actin cytoskeleton dynamics by phosphorylating and inactivating cofilin, an actin-depolymerizing protein. This activity influences cell motility, morphogenesis, and synaptic plasticity. LIMK1 is activated by upstream Rho GTPase signaling pathways, including RhoA/ROCK and Rac1/PAK, linking extracellular signals to cytoskeletal reorganization. Dysregulation of LIMK1 has been implicated in cancer metastasis, neurological disorders (e.g., Alzheimer’s disease), and developmental defects, making it a target for therapeutic research.

LIMK1 antibodies are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect LIMK1 in tissues or cultured cells. Specific antibodies can distinguish between phosphorylated (active) and total LIMK1. aiding in pathway activation studies. Validated LIMK1 antibodies are critical for ensuring specificity, as cross-reactivity with LIMK2 (a closely related homolog) may occur. Commercial antibodies are often raised against unique epitopes within the LIM or kinase domains. Research applications include exploring LIMK1’s role in tumor invasiveness, neuronal development, and its interplay with signaling cascades, supporting drug discovery efforts targeting LIMK1-associated diseases.