CytochromeP4504A抗体;CytochromeP4504A Antibody—艾普蒂 新品

+ +

•  

   

     Western blot analysis of Cytochrome P450 4A expression in Human fetal kidney lysate.    

  
  

Cytochrome P450 4A (CYP4A) antibodies are immunological tools used to detect and study CYP4A enzymes, a subfamily of the cytochrome P450 monooxygenase superfamily. These enzymes are primarily involved in the metabolism of fatty acids, particularly the ω-hydroxylation of arachidonic acid and other eicosanoids, playing critical roles in blood pressure regulation, vascular function, and lipid homeostasis. In mammals, CYP4A isoforms (e.g., CYP4A1. CYP4A2. CYP4A3. and CYP4A8 in rodents; CYP4A11 and CYP4A22 in humans) are expressed in tissues such as the liver, kidney, and vasculature. Dysregulation of CYP4A activity has been linked to hypertension, renal disease, and metabolic syndromes, often through the production of bioactive lipid mediators like 20-hydroxyeicosatetraenoic acid (20-HETE).

CYP4A antibodies are essential for identifying enzyme localization, expression levels, and activity in experimental models. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate tissue-specific CYP4A distribution under physiological or pathological conditions. Some antibodies exhibit cross-reactivity across species (e.g., human, rat, mouse), facilitating comparative studies. Researchers also utilize these antibodies to explore CYP4A's role in oxidative stress, inflammation, and drug metabolism, particularly in contexts like ischemia-reperfusion injury, diabetes, and cardiovascular diseases. Validation of antibody specificity via knockout controls or enzymatic assays is critical to ensure accurate interpretation in research applications.