DNAPolymerasegamma抗体;DNAPolymerasegamma Antibody—艾普蒂 新品

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     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P12574(GABRB1 Antibody) at dilution 1/25. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P12574(GABRB1 Antibody) at dilution 1/25. (Original magnification: ×200)    

  
  

DNA polymerase gamma (Pol γ) antibody is a crucial tool in studying mitochondrial DNA (mtDNA) replication and repair. Pol γ, encoded by the *POLG* gene, is the sole DNA polymerase responsible for replicating and repairing mtDNA in eukaryotic cells. It consists of a catalytic subunit (Pol γA) and an accessory subunit (Pol γB), which together ensure high-fidelity synthesis of mtDNA. Mutations in *POLG* are linked to mitochondrial disorders such as Kearns-Sayre syndrome, Alpers syndrome, and progressive external ophthalmoplegia, highlighting its essential role in mitochondrial function.

Antibodies targeting Pol γ are typically generated using recombinant proteins or synthetic peptides corresponding to conserved regions of Pol γA or Pol γB. These antibodies enable the detection, quantification, and localization of Pol γ in various tissues and cell types through techniques like Western blotting, immunohistochemistry, and immunofluorescence. They are vital for diagnosing *POLG*-related diseases, assessing protein expression levels in mitochondrial dysfunction studies, and investigating the impact of pathogenic mutations on Pol γ stability or activity.

Additionally, Pol γ antibodies support research into mtDNA maintenance mechanisms, aging, and chemotherapy-induced mitochondrial toxicity. Their specificity is often validated using knockout cell lines or competitive peptide-blocking assays. By facilitating the study of Pol γ's structure-function relationships and its interactions with other mitochondrial proteins, these antibodies contribute to advancing our understanding of mitochondrial biology and associated diseases.