p150CAF1抗体;p150CAF1 Antibody—艾普蒂 新品

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     Western blot analysis of p150 CAF1 expression in K562 cell lysate.    

  
  

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

The p150CAF1 antibody targets the p150 subunit of the CCR4-NOT complex, a highly conserved multi-protein assembly critical for post-transcriptional gene regulation in eukaryotic cells. Originally identified in yeast, the CCR4-NOT complex plays diverse roles in mRNA metabolism, including deadenylation (removal of poly(A) tails), translation repression, and transcriptional regulation. The p150 subunit (also called CNOT1) serves as a scaffold protein that stabilizes the complex and coordinates interactions with other subunits, deadenylases (e.g., CNOT6/6L, CNOT7/8), and RNA-binding proteins.

Studies using p150CAF1 antibodies have been instrumental in elucidating the complex's involvement in cellular processes such as cell cycle progression, stress responses, and differentiation. The antibody is widely used in techniques like immunoprecipitation, Western blotting, and immunofluorescence to investigate CCR4-NOT complex localization, protein interactions, and expression dynamics. Dysregulation of the CCR4-NOT complex, including p150CAF1. has been linked to cancer, neurological disorders, and viral infection responses, making this antibody a valuable tool for both basic research and disease mechanism studies. Commercial p150CAF1 antibodies are typically raised against specific epitopes in human or model organism proteins, with validation across experimental applications.