Phospho-UBF1(S484)抗体;Phospho-UBF1(S484) Antibody—艾普蒂 新品

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

The Phospho-UBF1 (S484) antibody is designed to detect UBF1 (Upstream Binding Factor 1) when phosphorylated at serine 484. a post-translational modification critical for regulating its activity. UBF1. a nucleolar transcription factor, plays a key role in RNA polymerase I-mediated transcription of ribosomal RNA (rRNA) genes, essential for ribosome biogenesis and cell growth. Phosphorylation at S484 occurs during specific phases of the cell cycle, particularly during the G1/S transition, and is mediated by cyclin-dependent kinases (CDKs) or extracellular signal-regulated kinases (ERKs). This modification modulates UBF1’s ability to bind rDNA promoters, recruit transcriptional machinery, or interact with chromatin-remodeling complexes, thereby influencing rRNA synthesis rates.

The antibody is widely used in studies investigating nucleolar dynamics, ribosome biogenesis, and cell proliferation. It enables researchers to assess UBF1 activation status in response to growth signals, stress, or pharmacological inhibitors. Applications include Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to map UBF1’s occupancy on rDNA under varying phosphorylation conditions. Dysregulation of UBF1 phosphorylation has been implicated in cancers, aging, and ribosomopathies, making this antibody a valuable tool for both basic and translational research. Specific validation via knockout/knockdown controls or phosphatase treatment is recommended to confirm signal specificity.