KDM5A抗体;KDM5A Antibody—艾普蒂

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     Western blot analysis of KDM5A / Jarid1A / RBBP2 expression in (1) HEK293 cell lysate; (2) Mouse spleen lysate.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

The KDM5A antibody is a crucial tool in studying the lysine-specific demethylase 5A (KDM5A), also known as JARID1A or RBP2. which belongs to the KDM5 subfamily of histone demethylases. KDM5A specifically catalyzes the removal of methyl groups from tri- and dimethylated histone H3 lysine 4 (H3K4me3/me2), a modification typically associated with active gene transcription. By regulating H3K4 methylation states, KDM5A modulates chromatin structure and gene expression, impacting processes like cell differentiation, proliferation, and DNA repair. Dysregulation of KDM5A has been linked to cancers, where its overexpression often correlates with poor prognosis, drug resistance, and epigenetic silencing of tumor suppressors.

Antibodies targeting KDM5A are widely used in research to detect its expression, localization, and functional interactions. These antibodies enable techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP-seq), aiding in the exploration of its role in epigenetic regulation and disease mechanisms. Validated KDM5A antibodies are essential for distinguishing it from homologous family members (KDM5B/C/D) due to structural similarities within the JmjC domain-containing protein family.

High-quality KDM5A antibodies are rigorously tested for specificity, often using knockout cell lines or siRNA knockdowns. They are critical in both basic research and drug development, particularly in studies targeting KDM5A's oncogenic functions or its potential as a therapeutic biomarker.