Cellubrevin抗体;Cellubrevin Antibody—艾普蒂 新品

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     Western blot analysis of Cellubrevin expression in A375 cell lysate.    

  
  

Cellubrevin, also known as vesicle-associated membrane protein 3 (VAMP3), is a member of the SNARE protein family that plays a critical role in intracellular membrane fusion events, particularly in endocytic and recycling pathways. It mediates vesicle docking and fusion by forming complexes with target membrane SNAREs (t-SNAREs) like syntaxin and SNAP-25. Unlike other VAMP isoforms (e.g., VAMP1/2 in neuronal cells), cellubrevin is ubiquitously expressed in non-neuronal tissues and regulates processes such as phagocytosis, cytokine secretion, and GLUT4 translocation in adipocytes.

Antibodies targeting cellubrevin are essential tools for studying its localization, expression, and function in cellular trafficking. Developed using immunogens derived from conserved regions of the protein (e.g., N-terminal or cytoplasmic domains), these antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation. They help identify cellubrevin's involvement in pathological conditions, including metabolic disorders, immune dysregulation, and cancer metastasis, where vesicle trafficking is disrupted. For instance, studies using cellubrevin antibodies have revealed its role in tumor cell invasion by regulating integrin recycling. Validation of antibody specificity often involves knockout cell lines or peptide-blocking assays to ensure minimal cross-reactivity with homologous VAMP proteins. Such antibodies remain pivotal in elucidating membrane dynamics in both physiological and disease contexts.