HES5抗体;HES5 Antibody—艾普蒂

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

The HES5 antibody is a research tool used to detect HES5. a transcription factor belonging to the HES (hairy and enhancer of split) family, which plays critical roles in developmental processes. HES5 is primarily involved in the Notch signaling pathway, a conserved system regulating cell differentiation, proliferation, and stem cell maintenance. It acts as a transcriptional repressor, binding to DNA through its basic helix-loop-helix (bHLH) domain to inhibit the expression of target genes, including those promoting neuronal differentiation. This makes HES5 essential in neural development, where it maintains neural progenitor cells in an undifferentiated state.

HES5 expression is tightly regulated and often transient, peaking during embryonic development. Dysregulation of HES5 has been linked to developmental disorders and cancers, such as gliomas and neuroblastomas, where its overexpression may contribute to tumorigenesis or therapy resistance.

The HES5 antibody is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study protein expression patterns in tissues or cultured cells. It helps researchers explore HES5’s role in neural stem cell dynamics, organogenesis, and cancer biology. Validation of antibody specificity is crucial, as cross-reactivity with other HES family members (e.g., HES1) could lead to misinterpretation. Commercial HES5 antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes within the protein’s N-terminal or bHLH regions. Its application advances understanding of developmental pathways and disease mechanisms.