TRAP抗体;TRAP Antibody—艾普蒂

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     Western blot analysis of Tartrate Resistant Acid Phosphatase expression in A431 cell lysate.    

  
  

TRAP (Tartrate-Resistant Acid Phosphatase) antibodies are immunological tools used to detect the TRAP enzyme, a metalloproteinase highly expressed in osteoclasts and certain immune cells. TRAP, also known as acid phosphatase 5 (ACP5), exists as two isoforms: 5a (secreted) and 5b (osteoclast-specific). It plays critical roles in bone resorption, immune regulation, and iron metabolism. TRAP antibodies are particularly valuable in biomedical research and diagnostics. In bone biology, they help identify osteoclast activity, aiding studies on osteoporosis, bone metastases, and rare skeletal disorders. In hematopathology, TRAP antibodies assist in diagnosing hairy cell leukemia (HCL), where TRAP isoform 5a is overexpressed in malignant B cells.

These antibodies are commonly utilized in techniques like immunohistochemistry (IHC), western blotting, and flow cytometry. Monoclonal TRAP antibodies offer high specificity, distinguishing TRAP from other acid phosphatases due to its unique resistance to tartrate inhibition. Recent studies also explore TRAP's involvement in macrophage polarization and fibrosis, expanding its diagnostic relevance. Clinically, serum TRAP 5b levels serve as a biomarker for bone turnover rates, while TRAP 5a correlates with HCL progression.

Despite their utility, TRAP antibody applications require careful validation, as enzyme activity and epitope accessibility can vary across sample types. Ongoing research aims to refine TRAP-targeted therapies and improve assay standardization.