CRALBP抗体;CRALBP Antibody—艾普蒂

+ +

•  

   

     Gel: 8%SDS-PAGE, Lysate: 50 μg, Lane 1-2: RAW264.7, NIH/3T3 cells, Primary antibody: P00348(EGR1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes    

  
  

Cellular retinaldehyde-binding protein (CRALBP), encoded by the *RLBP1* gene, is a crucial component in the vertebrate visual cycle. It facilitates the transport and isomerization of 11-cis-retinoids, essential for regenerating visual pigments in photoreceptor cells. CRALBP is predominantly expressed in retinal pigment epithelium (RPE) and Müller glial cells, where it stabilizes hydrophobic retinoids and ensures efficient recycling of visual chromophores.

Antibodies targeting CRALBP are widely used in ophthalmic research to investigate retinal diseases such as retinitis pigmentosa, macular degeneration, and Bothnia-type retinal dystrophy, which are linked to *RLBP1* mutations. These antibodies enable localization and quantification of CRALBP in tissue sections (via immunohistochemistry) or protein extracts (via Western blotting), helping to assess its expression under pathological conditions. They also aid in studying CRALBP's role in maintaining retinal homeostasis and its interactions with enzymes like RPE65.

Commercial CRALBP antibodies are typically raised in rabbits or mice using recombinant protein fragments or synthetic peptides. Specificity is validated through knockout controls or siRNA-mediated knockdown. Their applications extend to diagnostic research, drug development, and mechanistic studies targeting inherited retinal disorders. However, variability in antibody performance across experimental setups necessitates careful validation for reproducibility.