OGT抗体;OGT Antibody—艾普蒂

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     Western blot analysis of OGT / O-Linked N-Acetylglucosamine Transferase expression in A549 cell lysate.    

  
  

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     Immunofluorescent analysis using the Antibody at 1:50 dilution.    

  
  

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     Immunofluorescent analysis using the Antibody at 1:50 dilution.    

  
  

O-GlcNAc transferase (OGT) is an essential enzyme that catalyzes the addition of O-linked β-N-acetylglucosamine (O-GlcNAc) modifications to serine and threonine residues of target proteins. This post-translational modification, termed O-GlcNAcylation, plays a critical role in regulating cellular processes such as nutrient sensing, transcription, stress response, and epigenetic regulation. As a key mediator of the hexosamine biosynthetic pathway, OGT links metabolic flux to cellular signaling by dynamically modifying nuclear, cytoplasmic, and mitochondrial proteins. Dysregulation of OGT activity or O-GlcNAc cycling has been implicated in metabolic disorders, cancer, neurodegenerative diseases, and cardiovascular conditions.

OGT antibodies are indispensable tools for studying the enzyme's expression, localization, and functional interactions. They enable detection of OGT protein levels via Western blotting, immunohistochemistry, or immunofluorescence, and facilitate research into its substrate specificity and regulatory mechanisms. Due to OGT's structural complexity (including multiple isoforms and a tetratricopeptide repeat domain for protein interactions), generating specific antibodies requires careful validation to avoid cross-reactivity. These antibodies have advanced understanding of OGT's dual roles as a nutrient sensor and epigenetic modulator, particularly its interplay with phosphorylation signaling and involvement in insulin resistance, aging, and chromatin remodeling. Recent studies also explore OGT's potential as a therapeutic target, emphasizing the continued importance of high-quality antibodies in both basic and translational research.