SFPQ抗体;SFPQ Antibody—艾普蒂

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     Western blot analysis of SFPQ expression in A431 cell lysate.    

  
  

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     All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

The SFPQ (Splicing Factor Proline and Glutamine Rich) antibody is a key tool for studying the multifunctional SFPQ protein, also known as PSF (PTB-associated Splicing Factor). SFPQ is a ubiquitously expressed RNA/DNA-binding protein involved in critical cellular processes, including pre-mRNA splicing, transcriptional regulation, DNA repair, and paraspeckle formation. It often forms heterodimers with NONO or PSPC1. contributing to large molecular complexes (∼700-800 kDa) that mediate RNA processing and genomic stability. SFPQ is essential for neuronal development, immune responses, and cell cycle control, with dysregulation linked to neurodegenerative diseases (e.g., ALS), viral infections, and cancers.

SFPQ antibodies, typically monoclonal or polyclonal, are widely used in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate its localization, expression levels, and interactions. These antibodies help identify SFPQ’s dual roles in gene expression—acting as a splicing repressor or activator depending on cellular context—and its involvement in DNA damage response pathways. Researchers also utilize SFPQ antibodies to explore its pathological roles, such as forming toxic aggregates in ALS or modulating oncogenic signaling in tumors. Validated specificity for target epitopes ensures reliability in both basic research and clinical studies aiming to dissect SFPQ-related molecular mechanisms or therapeutic targets.