Palladin抗体;Palladin Antibody—艾普蒂

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Western blot analysis using PSMC3 mouse mAb against COS7 (1), C6 (2), Hela (3), and A431 (4) cell lysate.;COS7,C6,Hela,A431    

  
  

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     Immunofluorescence analysis of MCF-7 cells using PSMC3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of Hela cells using PSMC3 mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using PSMC3 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using PSMC3 mouse mAb with DAB staining.    

  
  

Palladin antibodies are essential tools in studying the Palladin protein, a cytoskeleton-associated molecule implicated in cell adhesion, motility, and structural organization. Discovered in the early 2000s, Palladin is expressed as multiple isoforms (e.g., 90 kDa, 140 kDa) generated by alternative splicing, with roles in actin filament bundling and focal adhesion dynamics. It interacts with proteins like VASP and α-actinin, influencing cell migration and mechanical signaling. Dysregulation of Palladin has been linked to pathological processes, particularly cancer metastasis, where its overexpression in tumors (e.g., pancreatic, breast) correlates with enhanced invasiveness. Research also associates Palladin mutations with hereditary neurodevelopmental disorders.

Palladin antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to detect expression patterns, subcellular localization, and isoform-specific functions. Their development required careful epitope targeting due to isoform diversity, with many antibodies validated against specific regions (e.g., immunoglobulin-like domains). Challenges include ensuring specificity, as cross-reactivity with paralogs (e.g., myotilin) may occur. Recent studies utilize Palladin antibodies to explore its role in tumor microenvironments, fibroblast activation, and mechanotransduction pathways. These reagents remain critical for unraveling Palladin's dual physiological and pathological roles, offering potential diagnostic or therapeutic insights in cancer and connective tissue diseases.