TFIIEalpha抗体;TFIIEalpha Antibody—艾普蒂 新品

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

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     Western blot analysis of TFIIE alpha expression in HeLa cell lysate.    

  
  

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     Immunofluorescent analysis using the Antibody at 1:50 dilution.    

  
  

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     Immunofluorescent analysis using the Antibody at 1:150 dilution.    

  
  

The TFIIEα antibody is a crucial tool in molecular biology for studying the role of TFIIEα, a subunit of the general transcription factor TFIIE, which is essential for RNA polymerase II-mediated transcription. TFIIE, composed of α (TFIIEα) and β (TFIIEβ) subunits, facilitates transcription initiation by stabilizing the pre-initiation complex, promoting TFIIH kinase activity, and regulating promoter escape. Specifically, TFIIEα interacts with RNA polymerase II and other transcription factors, bridging promoter recognition and the transition to elongation. Its dynamic involvement in DNA repair and transcription-coupled repair further highlights its functional versatility.

Antibodies targeting TFIIEα enable researchers to investigate its expression, localization, and interactions in diverse experimental contexts, including Western blotting, immunoprecipitation, chromatin immunoprecipitation (ChIP), and immunofluorescence. These applications help elucidate TFIIEα's role in transcriptional regulation, cell cycle progression, and stress responses. Dysregulation of TFIIEα has been implicated in diseases like cancer and developmental disorders, making its study clinically relevant. For instance, altered TFIIEα expression may disrupt transcription fidelity, contributing to oncogenesis. The antibody's specificity ensures accurate detection, aiding mechanistic studies of gene expression control and potential therapeutic targeting. Commercial TFIIEα antibodies are typically validated across species (e.g., human, mouse) and sample types (cells, tissues), supporting broad research utility in both basic and translational biomedical fields.