Nucleolin抗体;Nucleolin Antibody—艾普蒂 新品

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     Western blot analysis of Nucleolin expression in K562 cell lysate.    

  
  

Nucleolin is a multifunctional phosphoprotein predominantly located in the nucleolus, though it also shuttles to the cytoplasm and cell membrane. It plays critical roles in ribosome biogenesis, chromatin organization, RNA metabolism, and regulation of cell proliferation, apoptosis, and stress responses. Structurally, it contains three conserved domains: an N-terminal acidic region with phosphorylation sites, central RNA-binding domains, and a C-terminal RGG motif for RNA interactions. Dysregulation of nucleolin is linked to cancer, viral infections, and autoimmune diseases, with overexpression observed in many tumors, making it a potential therapeutic target.

Nucleolin antibodies are essential tools for studying its expression, localization, and function. These antibodies are produced by immunizing hosts (e.g., mice, rabbits) with nucleolin-derived antigens, such as full-length proteins or specific epitopes. They enable detection via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Specificity varies depending on the antibody clone; some recognize conserved regions across species, while others target post-translational modifications or disease-associated variants. Validation includes knockout controls and cross-reactivity assessments. Applications range from basic research on ribosome assembly to clinical studies exploring nucleolin's role in cancer progression or as a biomarker. Challenges include distinguishing nucleolin isoforms and ensuring consistent performance across experimental conditions.