Nesprin3抗体;Nesprin3 Antibody—艾普蒂 新品

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     Western blot analysis of Nesprin3 expression in 293 cell lysate.    

  
  

Nesprin3 (nuclear envelope spectrin repeat protein 3) is a member of the nesprin family, which plays a critical role in maintaining nuclear architecture and mediating connections between the nucleus and the cytoskeleton. Anchored at the nuclear envelope via its C-terminal KASH domain, Nesprin3 interacts with SUN proteins to form LINC (Linker of Nucleoskeleton and Cytoskeleton) complexes. These complexes facilitate mechanical coupling between the nuclear lamina and cytoplasmic filaments, influencing processes like nuclear positioning, cell migration, and mechanotransduction. Nesprin3 specifically associates with the actin cytoskeleton through its N-terminal domain, distinguishing it from other nesprins that bind intermediate filaments or microtubules. Its expression is implicated in tissue development, wound healing, and cancer metastasis, where cellular mechanics are pivotal.

Antibodies targeting Nesprin3 are essential tools for studying its localization, interactions, and functions. They enable detection via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Validation of these antibodies typically involves knockout controls or siRNA knockdown to confirm specificity, given potential cross-reactivity with other nesprin isoforms. Research utilizing Nesprin3 antibodies has revealed its dynamic redistribution during cell polarization and its role in nuclear deformation under mechanical stress. Dysregulation of Nesprin3 has been linked to pathologies including muscular dystrophies, cardiomyopathies, and metastatic cancers, making these antibodies valuable for both basic research and clinical investigations into nuclear-cytoskeletal crosstalk in disease mechanisms.